@article{Veronesi2024b,

title = {(±)‐Catechins inhibit prehaustorium formation in the parasitic weed <scp>\textit{Phelipanche ramosa}</scp> and reduce tomato infestation},

author = {Christophe Veronesi and Estelle Billard and Philippe Delavault and Philippe Simier},

doi = {10.1002/ps.8472},

issn = {1526-4998},

year  = {2024},

date = {2024-10-05},

urldate = {2024-10-05},

journal = {Pest Management Science},

publisher = {Wiley},

abstract = {<jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p><jats:italic>Phelipanche ramosa</jats:italic> L. (Pomel) is a noxious parasitic weed in field and vegetable crops in Mediterranean countries. Control of this pest is complex and far from being achieved, and new environmentally‐friendly strategies are being sought. The present study evaluates the possibility of using (±)‐catechins as a natural herbicide against broomrapes.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>The results show that (±)‐catechins have no effect on GR24‐induced germination over a wide concentration range (10<jats:sup>−4</jats:sup> to 10<jats:sup>−10</jats:sup> <jats:sc>m</jats:sc>), nor on radicle elongation after germination, but strongly inhibit, at 10<jats:sup>−4</jats:sup> and 10<jats:sup>−5</jats:sup> <jats:sc>m</jats:sc>, prehaustorium formation in response to the haustorium‐inducing factor, <jats:italic>cis/trans</jats:italic>‐zeatin. Accordingly, pot experiments involving the supplies of 10<jats:sup>−5</jats:sup> <jats:sc>m</jats:sc> of (±)‐catechins to tomato plants infested or not with <jats:italic>P</jats:italic>. <jats:italic>ramosa</jats:italic> demonstrate that (±)‐catechins do not influence growth of non‐parasitized tomato plants and prevent heavy infestation by strongly reducing parasite attachments and inducing parasite necrosis once they are attached.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p>This study points the potential use of (±)‐catechins for parasitic weed control. It raises also the question of the mechanisms involved in the inhibition of prehaustorium formation and the necrosis of parasite attachments in response to (±)‐catechins application. © 2024 The Author(s). <jats:italic>Pest Management Science</jats:italic> published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.</jats:p></jats:sec>},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{Grandjean2024d,

title = {Pectin Remodeling and Involvement of AtPME3 in the Parasitic Plant–Plant Interaction, Phelipanche ramosa–Arabidospis thaliana},

author = {Cyril Grandjean and Christophe Veronesi and Christine Rusterucci and Charlotte Gautier and Yannis Maillot and Maïté Leschevin and Françoise Fournet and Jan Drouaud and Paulo Marcelo and Luciane Zabijak and Philippe Delavault and Philippe Simier and Sophie Bouton and Karine Pageau},

doi = {10.3390/plants13152168},

issn = {2223-7747},

year  = {2024},

date = {2024-08-00},

urldate = {2024-08-00},

journal = {Plants},

volume = {13},

number = {15},

publisher = {MDPI AG},

abstract = {<jats:p>Phelipanche ramosa is a root parasitic plant fully dependent on host plants for nutrition and development. Upon germination, the parasitic seedling develops inside the infected roots a specific organ, the haustorium, thanks to the cell wall-degrading enzymes of haustorial intrusive cells, and induces modifications in the host’s cell walls. The model plant Arabidopsis thaliana is susceptible to P. ramosa; thus, mutants in cell wall metabolism, particularly those involved in pectin remodeling, like Atpme3-1, are of interest in studying the involvement of cell wall-degrading enzymes in the establishment of plant–plant interactions. Host–parasite co-cultures in mini-rhizotron systems revealed that parasite attachments are twice as numerous and tubercle growth is quicker on Atpme3-1 roots than on WT roots. Compared to WT, the increased susceptibility in AtPME3-1 is associated with reduced PME activity in the roots and a lower degree of pectin methylesterification at the host–parasite interface, as detected immunohistochemically in infected roots. In addition, both WT and Atpme3-1 roots responded to infestation by modulating the expression of PAE- and PME-encoding genes, as well as related global enzyme activities in the roots before and after parasite attachment. However, these modulations differed between WT and Atpme3-1, which may contribute to different pectin remodeling in the roots and contrasting susceptibility to P. ramosa. With this integrative study, we aim to define a model of cell wall response to this specific biotic stress and indicate, for the first time, the role of PME3 in this parasitic plant–plant interaction.</jats:p>},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN23,

title = {New Insights into Phloem Unloading and Expression of Sucrose Transporters in Vegetative Sinks of the Parasitic Plant Phelipanche ramosa L. (Pomel)},

author = {Thomas Péron and Adrien Candat and Grégory Montiel and Christophe Veronesi and David Macherel and Philippe Delavault and Philippe Simier},

url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5220101/pdf/fpls-07-02048.pdf},

doi = {10.3389/fpls.2016.02048},

issn = {1664-462X (Print) 1664-462x},

year  = {2016},

date = {2016-01-01},

urldate = {2016-01-01},

journal = {Front Plant Sci},

volume = {7},

pages = {2048},

abstract = {The plant-parasitic plant interaction is a interesting model to study sink-source relationship and phloem unloading. The parasitic plants, such as the achlorophyllous plant Phelipanche ramosa, connect to the host phloem through the haustorium and act as supernumerary sinks for the host-derived photoassimilates, primarily sucrose. The application of the fluorescent symplastic tracer, carboxyfluorescein (CF) derived from carboxyfluorescein diacetate (CFDA), to the leaves of the host plant (Brassica napus) showed direct phloem connections at the host-parasite interface. These experiments also evidenced the dominant apoplastic pathway for phloem unloading in major vegetative sinks of the parasite, including tubercles and shoots, except the adventitious root apices. The CF experiments showed also the symplastic isolation of the phloem tissues from the sink tissues in tubercle and shoot of the parasite, then suggesting the pivotal role of sucrose transporters in sucrose unloading in P. ramosa sinks. Three cDNAs encoding sucrose transporters (PrSUT) were isolated from the parasitic plant. PrSUT1 transcripts accumulated at the same level in the tubercle throughout the parasite growth while a significant increase in transcript accumulation occurred after emergence in the flowering shoot, notably in the growing apical part. The in situ hybridization experiments revealed the PrSUT1 transcript accumulation in the mature phloem cells of both subterranean and flowering shoots, as well as in shoot terminal sinks corresponding to apical meristem, scale leaf primordia and immature vasculature. The transient expression experiments in Arabidopsis protoplasts showed that PrSUT1 was localized at the plasma membrane, suggesting its role in phloem functioning and sucrose uptake by the sink cells in P. ramosa. Conversely, the PrSUT2 transcript accumulation was constantly low in tubercles and shoots but PrSUT3 transcripts accumulated markedly in the subterranean and flowering shoots, in concordance with the PrSUT3 mRNA accumulation in multiple sink areas including apical meristem, scale-leaf primordia, immature vasculature and even storage parenchyma. However, the PrSUT3 transcripts did not accumulate in the mature phloem cells. The transient expression experiments in Arabidopsis protoplasts suggested a tonoplast localization of PrSUT3, for which nevertheless the involvement in intracellular sucrose transport needs clarification.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{RN22,

title = {Seed response to strigolactone is controlled by abscisic acid-independent DNA methylation in the obligate root parasitic plant, Phelipanche ramosa L. Pomel},

author = {Marc-Marie Lechat and Guillaume Brun and Grégory Montiel and Christophe Véronési and Philippe Simier and Séverine Thoiron and Jean-Bernard Pouvreau and Philippe Delavault},

url = {https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4449535/pdf/erv119.pdf},

doi = {10.1093/jxb/erv119},

issn = {0022-0957 (Print) 0022-0957},

year  = {2015},

date = {2015-01-01},

urldate = {2015-01-01},

journal = {J Exp Bot},

volume = {66},

number = {11},

pages = {3129-40},

abstract = {Seed dormancy release of the obligate root parasitic plant, Phelipanche ramosa, requires a minimum 4-day conditioning period followed by stimulation by host-derived germination stimulants, such as strigolactones. Germination is then mediated by germination stimulant-dependent activation of PrCYP707A1, an abscisic acid catabolic gene. The molecular mechanisms occurring during the conditioning period that silence PrCYP707A1 expression and regulate germination stimulant response are almost unknown. Here, global DNA methylation quantification associated with pharmacological approaches and cytosine methylation analysis of the PrCYP707A1 promoter were used to investigate the modulation and possible role of DNA methylation during the conditioning period and in the PrCYP707A1 response to GR24, a synthetic strigolactone analogue. Active global DNA demethylation occurs during the conditioning period and is required for PrCYP707A1 activation by GR24 and for subsequent seed germination. Treatment with 5-azacytidine, a DNA-hypomethylating molecule, reduces the length of the conditioning period. Conversely, hydroxyurea, a hypermethylating agent, inhibits PrCYP707A1 expression and seed germination. Methylated DNA immunoprecipitation followed by PCR experiments and bisulfite sequencing revealed that DNA demethylation particularly impacts a 78-nucleotide sequence in the PrCYP707A1 promoter. The results here demonstrate that the DNA methylation status during the conditioning period plays a crucial role independently of abscisic acid in the regulation of P. ramosa seed germination by controlling the strigolactone-dependent expression of PrCYP707A1.},

keywords = {},

pubstate = {published},

tppubtype = {article}

}